Fig. 4

Correlative studies on peripheral blood immune response. a UMAP visualisation of CD45⁺ immune cells and CD45⁺ cells were clustered on the basis of their high-dimensional expression profile using Cluster X. Each dot corresponds to a single cell. Representative data from 16 patients, for whom PBMC analysis was performed at C1D1 and C3D1 (C3D1 missing for one patient); subsampled up to 5000 per sample; analysis of 155,000 cells in total. b Box and whisker plots showing the percentage of mMDSC, inflammatory monocytes, classical monocytes and non-classical monocytes in CD45⁺ cells at C1D1 (n = 16) and C3D1 (n = 15). n.s, not significant, *p < 0.05; comparison using Wilcoxon matched-pairs test. c, Kaplan–Meier curves for progression-free survival with patients stratified according to mMDSC percentage at C1D1 with a cutoff at the median. Two-sided P value with significance level set at 0.05. d Box and whisker plots showing the percentage of CD4⁺ T, CD8⁺ T, conventional CD4⁺ T, Treg, naïve, central memory (CM), effector memory (EM), effector memory CD45RA⁺ (EMRA) CD8⁺ or CD4⁺ T, Treg HLA-DR⁺, conventional CD4⁺ HLA-DR⁺, CD8⁺ HLA-DR⁺, NKT, NK, NK CD56^dim CD16⁺ and NK CD56^bright CD16⁺ cells in CD45⁺ cells at C1D1 (n = 16) and C3D1 (n = 15). n.s. not significant; comparison using Wilcoxon matched-pairs test. e Kaplan–Meier curves for progression-free survival with patients stratified according to CD8⁺ T cells percentage at C1D1 with a cutoff at the median. Two-sided P value with significance level set at 0.05. f Kaplan–Meier curves for progression-free survival with patients stratified according to CD4⁺ HLA-DR⁺ T cells percentage at C3D1 with a cutoff at the median. Two-sided P value with significance level set at 0.05. g Parts of whole (red and blue) showing the percentage of positive (in red) or negative (in blue) antitumor responses against TERT or NY-ESO1 at C1D1 and C3J1 (n = 15). Survival distributions were compared using the log-rank test (c, e, f)