Fig. 4
From: Identification of glutamine as a potential therapeutic target in dry eye disease
Patient and rat samples suggested the potential involvement of glutaminase 1 (GLS1), an enzyme upstream of glutamine metabolism, in dry eye therapy. a Schematic illustration showing that GLS1 is a key enzyme to catalyze glutamine catabolism. b RNA-seq analysis of rat DED model suggested that D-glutamine and D-glutamate metabolism were enhanced in the dry eye group and gene GLS is up-regulated. c, d Immunohistochemical staining of clinical dry eye patients corroborated the significant increase of GLS1 in the cornea, n = 3. Scale bar = 50 μm. e, f A rat model of dry eye verified that GLS1 was up-regulated in the dry eye group, whereas the MSC + Tβ4 combination treatment reduced GLS1 expression, n = 3. Scale bar = 100 μm
