Fig. 3

CVB3 infection of cardiac structure cells and immune cells. a The stage delineation of disease progression based on Pearson correlation. b Volcano plot of regulated genes when compare the cells of early and late stages. c Dot plot shows the GO terms of specifically expressed genes in cardiomyocytes of each stage. d Violin plot showing the level of CVB3 in cells of control and CVB3-infected mice heart from 1 to 7 dpi in the snRNA-seq data. e The level of CVB3 in cardiac tissue sections from control and CVB3-infected mice from 1 to 7 dpi. f The proportion of CVB3 positive cells in all cell types in the snRNA-seq data. g The typical image of CVB3 mRNA distribution in the cardiac section. Scale bar: 500 μm. h Violin plot of CVB3 level in different spatial regions. i Bubble plot showing the level of CVB3 in each cell type of control and CVB3-infected mice hearts from 1 to 7 dpi in the snRNA-seq data. j Infection response score for cardiac structure cells in snRNA-seq data across control and CVB3 infected hearts from 1 to 7 dpi. k Representative spatial image of myocarditic region that distributed near the border region from CVB3-infected mice heart at 4 dpi. Scale bar: 500 μm. l Representative spatial image of the distance of myocarditic region to the border region of CVB3-infected mice heart at 4 dpi. Scale bar: 500 μm. m The distance of myocarditic region to the border region of CVB3-infected mice hearts from 1 to 7 dpi. n Immunofluorescence staining of MLSN and CVB3 showing infected mesothelial cells at 1 dpi. Scale bar: 50 μm. o Heatmap showing the number of signaling interaction among immune cells and structural cells in the early stage. p Violin plot showing the expression level of damage-associated molecular pattern genes in mesothelial cells of control and CVB3 infected mice at different stages. q Heatmap shows the expression level of toll-like receptors in major immure cell types. r Heatmap shows the interaction of the CSF signaling pathway among cardiac immune cells and structure cells. EC endothelial cell, Mac/mono macrophage/monocyte, FB fibroblast, CM cardiomyocyte, PC pericyte, T T cell, Endo endocardial cells, Neu neutrophil, SMC smooth muscle cell, LC lymphatic cell, B B cell, Mes mesothelial cell, NC neuron cell, Adipo adipocyte