Fig. 4

Macrophages chemotaxis activate cytotoxic T cells. a Spatial transcriptomic map of cardiac tissue section from CVB3-infected mice at 6 dpi colored by spot clusters representing transcriptionally distinct tissue regions. Scale bar: 500 μm. b Changes in average predicted fibroblast proportion across the infected ventricle. c Spatial distribution of fibroblast (left) and the expression of Bgn on the spatial section (right). d Box plot of ECM score among ventricle region, border zone and myocarditic region (left) and ECM score on the spatial section (right). e Changes in average predicted cell type proportions across the infected ventricle. f UMAP embedding of macrophages colored by manually annotated clusters. Box plot of pro-inflammatory (g) and chemokine (h) scores among different macrophage subclusters. Scores were calculated by AddModuleScore function of the Seurat package. i UMAP embedding shows the developmental trajectory of macrophage subclusters. j The densities of Mono_Ccr2, Mac_IFNIC and Inflammatory_Mac along the developmental trajectory. k The expression of Ccl3 and Cxcl2 along the developmental trajectory. l UMAP embedding of T cells colored by manually annotated clusters. m The number of T cell subpopulations during disease progression. n The functional gene sets and Ifng expression level in T cell subpopulations. o The interaction pathways between Inflammatory_Mac and Cd8⁺ effector T. p The spatial colocation of Inflammatory_Mac and Cd8⁺ effector T cells. Red means the abundance of Inflammatory_Mac, green means the abundance of Cd8⁺ effector T, yellow means the colocation of two cell types. Scale bar: 500 μm. Ven ventricle, BZ border zone, MR myocarditic region