Fig. 3
RBDXBB.1.5-HR vaccine induces strong cellular immune response in mice. a The representative images and quantitative analysis of IFN-γ-secreting lymphocytes in spleen after stimulation with peptides pools of XBB.1.5 and JN.1 full-length spike proteins (n = 5 mice per group). b, c the percentages of splenic IFN-γ- and TNF-α-secreting CD4+/CD8+ and CD44+ memory T cells after being stimulated by spike peptide pools covering full-length spike proteins of XBB.1.5 (b) and JN.1 (c) variants (n = 5 mice per group). The representative images and percentages of Tfh and GC B cells in inguinal lymph nodes (d, f) and spleen (e, g) tissues (n = 5 mice per group). Data are presented as mean with SEM. SFC, spot-forming cells; GC B, germinal center B cells; Tfh, T follicular helper cells. P values in (a–c) were conducted by Two-way ANOVA followed by Sidak’s multiple comparisons test, and in (d–g) were performed by Unpaired Student’s t-tests. ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05
