Fig. 1
MDH2 regulates cellular senescence. a Schematic diagram of ABPP. b Relative MDH2 and p16INK4a level in Blk and Dox-induced senescent MRC-5 cells. c Quantification of b. d Relative MDH2 and p16INK4a level in replicative senescent MEFs form P7 to P11. e Quantification of d. f SA-β-gal staining of sh-Scr/sh-Mdh2 MEFs (P11) transfected at P4. g Quantification of f. h Relative MDH2 and p16INK4a level in sh-Scr/sh-Mdh2 MEFs (P7) transfected at P4. i Quantification of h. j SA-β-gal staining of OE-NC/OE-Mdh2 MEFs (P10) transfected at P4. k Quantification of j. l Relative MDH2 and p16INK4a level in OE-NC/OE-Mdh2 MEFs (P8) transfected at P4. m Quantification of l. Error bars represent the standard deviation (± SEM.). The significance of differences of c, g, i, k, m was analyzed with two-sided Student’s t-test. The significance of difference of e was analyzed with Dunnett’s multiple comparisons tests (*p < 0.05, **p < 0.01, ***p < 0.005, n. s., not significant)
