Fig. 3 | Signal Transduction and Targeted Therapy

Fig. 3

From: Glibenclamide targets MDH2 to relieve aging phenotypes through metabolism-regulated epigenetic modification

Fig. 3

Gli alleviates cellular senescence dependent on MDH2. a Diagram of the MDH2 knockdown or overexpression assay in cells. b Relative p16INK4a level in sh-Scr/sh-Mdh2 MEFs (P7) treated with -/Gli (100 μM)/Met (100 μM) for 5 days. c Quantification of b. d SA-β-gal staining of sh-Scr/sh-Mdh2 MEFs (P7) treated with -/Gli (100 μM)/Met (100 μM) for 15 days. e Quantification of d. f Relative p16INK4a level in OE-NC/OE-Mdh2 MEFs (P7) treated with -/Gli (100 μM)/Met (100 μM) for 5 days. g Quantification of f. h SA-β-gal staining of OE-NC/OE-Mdh2 MEFs (P7) treated with -/Gli (100 μM)/Met (100 μM) for 15 days. i Quantification of h. Error bars represent the standard deviation (± SEM.). The significance of differences (*p < 0.05, **p < 0.01, ***p < 0.005, n. s., not significant) of all panels were analyzed with Tukey’s multiple comparations tests

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