Fig. 4

Gli targets the TCA cycle and regulates central carbon metabolism. a mtROS level in MEFs treated with -/Gli (100 μM)/LW6 (10 μM) for 2/4/8/12/24 h. b Relative fluorescence ratio indicating subcellular lactate level in H1299 cells treated with -/LW6 (10 μM)/Gli (100 μM)/oxamate (10 mM) for 2 h. c KEGG pathway analysis of differential metabolites in MRC-5 cells treated with -/Gli (100 μM) for 2 h. d Relative level of glycolysis intermediates in MRC-5 cells treated with -/Gli (100 μM) for 2 h. e Relative level of TCA cycle intermediates in MRC-5 cells treated with -/Gli (100 μM) for 2 h. f Relative level of amino acids in MRC-5 cells treated with -/Gli (100 μM) for 2 h. g KEGG pathway analysis of differential metabolites in MRC-5 cells treated with -/Gli (100 μM) for 24 h. h Relative level of glycolysis intermediates in MRC-5 cells treated with -/Gli (100 μM) for 24 h. i Relative level of TCA cycle intermediates in MRC-5 cells treated with -/Gli (100 μM) for 24 h. j Relative level of amino acids in MRC-5 cells treated with -/Gli (100 μM) for 24 h. For metabolome assays, 6 replicates were tested. Error bars represent the standard deviation (± SEM.). The significance of differences of a and b were analyzed with Tukey’s multiple comparisons tests, and other significance of differences in metabolome assays were analyzed with Sidak’s multiple comparisons tests (*p < 0.05, **p < 0.01, ***p < 0.005)