Fig. 6
MDH2 inhibition delays hepatic aging and induces H3K27me3 level in vivo. a Diagram of MDH2 knockdown assay in naturally aged mice (19-month). b AST level of mice in different groups. Mice at 2-month age were used in Young groups. c ALT level of mice in different groups. d HbA1c ratio in serum of mice in different groups. e Serum insulin level of mice in different groups. f Behavior index of hanging endurance in mice of different groups. g Total number of arm entries in Y-maze of mice in different groups. h Alteration rate in Y-maze of mice in different groups. i Relative MDH2 and p16INK4a level in mouse livers. j Quantification of i. k Sirius red staining of mouse livers. l Relative area of fibrosis in mouse livers, quantification of k. m Relative H3K4me3, H3K9me3 and H3K27me3 level in mouse livers of different groups (n = 5 for all groups). n Quantification of m. Young represents mice at 2-month age, and Old represents mice at 20.5-month age. Sample size for b-h: Young, n = 7; sh-Scr, n = 8; sh-Scr+Gli (10 mg/kg), n = 7; sh-Mdh2, n = 7; sh-Mdh2+Gli (10 mg/kg), n = 8. Sample size for i: n = 5 for all groups. Sample size for k: Young, n = 5; sh-Scr, n = 5; sh-Scr+Gli (10 mg/kg), n = 4; sh-Mdh2, n = 5; sh-Mdh2+Gli (10 mg/kg), n = 3. Error bars represent the standard deviation (± SEM.). Every dot in the plots presents the data of 1 mouse. The significance of differences (*p < 0.05, **p < 0.01, ***p < 0.005) of all panels were analyzed using Tukey’s multiple comparisons tests
