Fig. 1
From: A20 attenuates oxidized self-DNA-mediated inflammation in acute kidney injury
Self-DNA is oxidized and released in AKI. a Serum DNA value of healthy donors and active sepsis patients. (n = 9, mean ± SEM); ***P < 0.001. b Serum DNA values of mice treated with PBS, Cis, AA I, or LPS. (n = 5, mean ± SEM); ***P < 0.001. c Fluorescence microscopy of 8OH-dG (red) in kidneys of mice that were treated with PBS, Cis, AA I, or LPS (n = 3). scale bars, 10 μm. Schematic of genomic DNA isolation of renal tissue from mice that were intraperitoneally injected with cisplatin (d) (named Cis-DNA), AA I (e) (AA-DNA) or LPS (f) (LPS-DNA), and the specific time of implementing isolation was indicated in timeline. g 8OH-dG concentrations in DNA extracted from renal tissue of PBS, Cis, AA I, and LPS treated mice (n = 3, mean ± SEM); *P < 0.05, **P < 0.01, ***P < 0.001. h Fluorescence microscopy of DAPI (blue), 8OH-dG (green), and F4/80 (red) in kidneys of mice treated with Cis (n = 3). scale bars, 10 μm. i Fluorescence microscopy of DAPI (blue), 8OH-dG (green), and F4/80 (red) in kidneys of mice treated with LPS (n = 3). scale bars, 10 μm
