Fig. 3

Ox-self-DNA promotes the progression of AKI by facilitating pyroptosis. a Immunoblots for total and cleaved caspase-3, MLKL, and phosphorylated MLKL, total and cleaved GSDMD from BMDMs treated with PBS, PBS-DNA, Cis-DNA, or AA-DNA. IL-1β level of supernatant from BMDMs treated with PBS, PBS-DNA, Cis-DNA, or AA-DNA was analyzed by ELISA (b), with cell death determined by LDH release (c), (n = 3, mean ± SEM); ***P < 0.001. d Survival of mice injected with Cis plus PBS or Cis plus disulfiram (n = 10 per group). e Serum creatinine level of mice as described in d. (n = 5. mean ± SEM); **P < 0.01. f Survival of mice injected with AA I plus PBS or AA I plus disulfiram (n = 10 per group). g Serum creatinine level of mice as described in (f). (n = 5. mean ± SEM); *P < 0.05. h Immunoblots for NEK7 from BMDMs treated with PBS or various DNA. i Immunoblots for total and cleaved GSDMD from iBMDMs treated with PBS-DNA or AA-DNA with or without MCC950 treatment. j Survival of mice treated with Cis and intraperitoneally preinjected with PBS or MCC950 (n = 10 per group). k Serum creatinine level of mice as described in (j). (n = 5. mean ± SEM); *P < 0.05. l Survival of mice treated with AA I and intraperitoneally preinjected with PBS or MCC950 (n = 10 per group). m Serum creatinine level of mice as described in (l). (n = 5. mean ± SEM); **P < 0.01