Fig. 4
From: A20 attenuates oxidized self-DNA-mediated inflammation in acute kidney injury
A20 is a key molecule in response to ox-DNA. RNA-Seq was performed on mRNA isolated from ox-dsDNA90- or CDNs-treated BMDMs. a, d Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis demonstrated the upregulated pathways. Heatmaps of differentially expressed genes reactive to ox-dsDNA90 (b) or CDNs (e) stimulation. Volcano Plot of differential gene expression of ox-dsDNA90- (c) or CDNs- (f) treated BMDMs. Immunoblots for A20 from iBMDMs respectively treated with ox-dsDNA90 (g), LPS-DNA (h) or CDNs (i). j Quantitative PCR analysis of Tnfaip3 mRNA in iBMDMs treated with PBS, BAY11-7082 (1 μM), C-176 (1 μM), ox-dsDNA90, ox-dsDNA90 along with BAY11-7082 (1 μM, 4 h pretreated before ox-dsDNA90 stimulation) or ox-dsDNA90 along with C-176 (1 μM, 24 h pretreated before ox-dsDNA90 stimulation). (n = 3. mean ± SEM); **P < 0.01, ***P < 0.001. k Quantitative PCR analysis of Tnfaip3 mRNA in iBMDMs treated with indicated reagents. (n = 3, mean ± SEM); ***P < 0.001. l Immunoblots for A20, NF-κB-p65, and phosphorylated NF-κB-p65 from iBMDMs treated as described in (j)
