Fig. 5
From: A20 attenuates oxidized self-DNA-mediated inflammation in acute kidney injury
A20 inhibits STING activation. a Immunoblots for STAT1 and phosphorylated STAT1, TBK1 and phosphorylated TBK1, IRF3 and phosphorylated IRF3 from A20f/f BMDMs and A20myel-KO BMDMs with or without ox-dsDNA90 treatment. Quantitative PCR analysis of Cxcl10 (b) and Ifn-β (c) mRNA in BMDMs isolated from A20f/f BMDMs and A20myel-KO BMDMs with or without treatment with ox-dsDNA90. (n = 3, mean ± SEM); ***P < 0.001. CXCL10 (d) and IFN-β (e) ELISA of supernatant from BMDMs as described in (a). (n = 3, mean ± SEM); **P < 0.01, ***P < 0.001. f Immunoblots for STAT1 and phosphorylated STAT1, TBK1, and phosphorylated TBK1, IRF3 and phosphorylated IRF3 from BMDMs infected with lentivirus encoding green fluorescent protein (GFP) (oe-CTRL) or mouse A20/GFP (oe-A20) with or without ox-dsDNA90 treatment. Quantitative PCR analysis of Cxcl10 (g) and Ifn-β (h) mRNA in oe-CTRL iBMDMs and oe-A20 iBMDMs with or without treatment with ox-dsDNA90. (n = 3, mean ± SEM); ***P < 0.001. CXCL10 (i) and IFN-β (j) ELISA of supernatant from oe-CTRL iBMDMs or oe-A20 iBMDMs treated as indicated in (c). (n = 3, mean ± SEM); **P < 0.01, ***P < 0.001
