Fig. 6
From: A20 attenuates oxidized self-DNA-mediated inflammation in acute kidney injury
A20 inhibits ox-DNA-induced pyroptosis and limits the progression of AKI. a BMDMs was isolated from A20f/f or A20myel-KO mice and then stimulated with PBS, ox-dsDNA90 (ox-DNA) or LPS plus ATP (LA). The total and cleaved GSDMD of these BMDMs were measured by immunoblotting. IL-1β (b) and LDH (c) levels from the supernatant of BMDMs as depicted in (a) were measured. (n = 3, mean ± SEM). ***P < 0.001. d Immunoblots for total and cleaved GSDMD from PBS, ox-dsDNA90 or LPS followed by ATP stimulated oe-CTRL iBMDMs or oe-A20 iBMDMs. IL-1β (e) level from the supernatant of iBMDMs as described in (d) were measured by ELISA, and cell death was determined by LDH (f). (n = 3, mean ± SEM). ***P < 0.001. g Survival of Cis treated A20f/f or A20myel-KO mice. (n = 10 per group). h Serum creatinine of Cis-treated A20f/f or A20myel-KO mice. (n = 5, mean ± SEM). *P < 0.05. i Immunoblots for A20 from renal tissue of mice treated with Anc80L65-CTRL or Anc80L65-A20 (# indicates the serial number of individual mouse). j Survival of mice treated with Anc80L65-CTRL plus Cis or Anc80L65-A20 plus Cis. (n = 10 per group). k Serum creatinine of mice as depicted in (j). (n = 5, mean ± SEM). **P < 0.01. l Immunoblots for total and cleaved GSDMD from BMDMs stimulated with PBS, ox-dsDNA90, or LPS followed by ATP with or without pretreatment of TNF-α. m Survival of mice treated with Cis, with or without pretreatment of TNF-α. (n = 10 per group). n Serum creatinine of mice as depicted in (m). (n = 5, mean ± SEM). **P < 0.01. o Survival of mice treated with AA I, with or without pretreatment of TNF-α. (n = 10 per group). p Serum creatinine of mice as depicted in (o). (n = 5, mean ± SEM). **P < 0.01
