Fig. 8

Targeting NEK7 can alleviate acute kidney injury. NEK7 knockout efficiency of myeloid cells from Nek7^f/f or Nek7^myel-KO mice were detected by quantitative PCR analysis (a) and immunoblotting (b). c Survival curves of the Nek7^f/f or Nek7^myel-KO mice that treated with Cis. (n = 10 per group). d Serum creatinine of mice as depicted in (c). (n = 5, mean ± SEM). ***P < 0.001. e Representative images of HE staining of the renal tissue sections. Scale bar, 50 μm. (n = 5). f Histological analysis of tubular injury of renal tissue sections as described in (c). (n = 5, mean ± SEM); ***P < 0.001. g Representative images of Masson staining of the renal tissue sections. Scale bar, 50 μm. (n = 5). h Immunoblots for NEK7 from renal tissue of Cis-treated mice that underwent in vivo NEK7 knockdown by siRNA. (n = 3) (# indicates the serial number of the individual mouse). i Survival of Cis-treated siCTRL mice or siNEK7 mice (n = 10). j Serum creatinine of mice as described in i. (n = 5, mean ± SEM). **P < 0.01. k Representative images of HE staining of renal tissue sections from Cis-treated siCTRL mice or siNEK7 mice. Scale bar, 50 μm, (n = 9). l Histological analysis of tubular injury of renal tissue sections as described in (i). (n = 9, mean ± SEM); ***P < 0.001. m Representative images of Masson staining of the renal tissue sections. Scale bar, 50 μm. (n = 5). n Survival curves of the mice that treated with Cis or Cis plus 12 h pretreated A20-derived-peptide(15 mg/kg). (n = 10 per group). o Serum creatinine of mice as described in (n) (n = 5, mean ± SEM). ***P < 0.001. p Representative images of HE staining of renal tissue sections from mice that were treated with Cis or Cis plus 12 h pretreated A20-derived-peptide. Scale bar, 50 μm, (n = 9). q Histological analysis of tubular injury of renal tissue sections as described in (n). (n = 9, mean ± SEM); ***P < 0.001. r Representative images of Masson staining of the renal tissue sections. Scale bar, 50 μm. (n = 5)