Fig. 6

Effects of Spp1 depletion in macrophage on ICI treatment in mouse model. a Schematic representation of the experimental design with ICI/IgG treatment in Spp1-cKO mice compared to Spp1-WT mice. b Line graph (left) depicts the volumetric progression of subcutaneous tumors across four mouse groups from the study depicted in (a). Two batches of experiments were performed with four mice in each group. The image (right) shows the comparison of excised tumors among four groups for one batch. Data are represented as mean ± SEM. c Representation of multiplex immunofluorescence (mIF) images display the staining of T cells and Spp1⁺ macrophages within the subcutaneous tumors across four groups as shown in (b). d Bar plot quantifies the mIF-based proportions of T cell subtypes within the subcutaneous tumors of the four mouse groups, as shown in (b). Each group comprises four tumor samples except the Spp-cKO/aPD1 group (n = 2 available for mIF), with data collected from two sections per tumor. e Paired cell flow cytometry panel (left) and box plot (right) depict the percentage of apoptotic MC38 cells co-cultured with the spleen-derived T cells across four groups. Each group consists of four mice. f Line graph (left) depicts the volumetric progression of subcutaneous tumors across six ATBs-treated mouse groups with ICI/IgG treatment in Spp1-cKO mice compared to Spp1-WT mice. The image (right) shows the comparison of excised tumors among six groups. Each group consists of four mice. Data are represented as mean ± SEM. g Representation of mIF images reveals the spatial distribution of T cells within the subcutaneous tumors across six groups, as shown in (f). h Bar plot demonstrates the proportions of T cell subtypes in subcutaneous tumors across six mouse groups, as shown in (f). Each group comprises four tumor samples, with data collected from two sections per tumor sample