Fig. 4

Global and myeloid deletion of CD38 ameliorated HIRI-induced pyroptosis in vivo. The protein expressions and the quantitative analysis of NLRP3, IL-18 and GSDMD-N and the mRNA expressions of NLRP3, Caspase-1 and IL-18 were examined by Western blot (a) and QPCR (b) in liver tissues of CD38^KO and CD38^fl/fl mice after HIRI, respectively. The protein expressions and the quantitative analysis of NLRP3, GSDMD-N and IL-18, and the mRNA expressions of NLRP3, Caspase-1 and IL-18 were determined by Western blot (c) and QPCR (d) in liver tissues from CD38^MKO and CD38^fl/fl mice after HIRI. Data are shown as means ± SEM, *p < 0.05, **p < 0.01and ***p < 0.001, n = 3–6 per group