Fig. 1 | Signal Transduction and Targeted Therapy

Fig. 1

From: Exosomal transfer of pro-pyroptotic miR-216a-5p exacerbates anthracycline cardiotoxicity through breast cancer-heart pathological crosstalk

Fig. 1

Coincubation with breast cancer cells aggravates DOX-induced pyroptosis in cardiomyocytes. Adult murine ventricular cardiomyocytes (AMVCs) and human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) were cocultured with breast cancer cells (BCCs) for 24 hours with 1 μM DOX treatment. a Schematic diagram of the coculture of BCCs with cardiomyocytes using transwell chambers. b–e Representative images of and statistics for cell viability after DOX treatment. Scale bar, 50 μm.; (f, g) lactate dehydrogenase (LDH) release and IL-18 levels were assessed using colorimetric methods in AMVCs (n = 5) and in (h and i) hiPSC-CMs (n = 5) (one-way ANOVA was used to compare all experimental groups to the DMSO group). “BCCs” indicates breast cancer cells, Directional arrows indicate exosome transfer between donor cells (4T1 or MDA-MB-231) and recipient cells (hiPSC-CMs or AMVCs). “N-BCC-EXOs” denotes normal breast cancer cell EXOs, and “D-BCC-EXOs” denotes DOX-induced breast cancer cell EXOs. “ns” indicates non-significant, and “DOX” indicates doxorubicin. Data are presented as Mean ± SD

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