Fig. 11 | Signal Transduction and Targeted Therapy

Fig. 11

From: Exosomal transfer of pro-pyroptotic miR-216a-5p exacerbates anthracycline cardiotoxicity through breast cancer-heart pathological crosstalk

Fig. 11

Human exosomes aggravate DOX-induced cardiomyocyte pyroptosis, an effect attenuated by a miR-216a-5p inhibitor. a EXOs were isolated from cardiomyopathy patients with adriamycin (Dis-EXOs) and matched healthy donors (N-EXOs). Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) were transfected with a miR-216a-5p inhibitor/NC for 24 hours and then treated with Dis-EXOs/N-EXOs or DOX/DMSO for 24 hours. b Cell viability was determined (n = 5). c LDH release (d) the levels of pyroptosis-related proteins (GSDMD-N, cleaved-CASP1, and NLRP3) were assessed by western blotting. e, f The levels of pyroptosis-related cytokines (IL-1β and IL-18) were assessed (n = 5). g Western blotting was used to assess ITCH and TXNIP levels after cells were treated with Dis-EXOs (n = 5). “DOX” indicates doxorubicin. The schematic diagram part was created using SMART - Servier Medical Art by Servier. Data are presented as means ± SD

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