Fig. 1

CRISPR kinase library screening combined with transcriptome sequencing identifies PKMYT1 as a key determinant of the Sensitivity of OS to DDP. a Schematic representation of the CRISPR kinase library screening protocol. b The mRNA expression of PKMYT1 in six paired OS and adjacent normal tissues. P values were assessed by the t test. c The expression of PKMYT1 was assessed in osteoblast-differentiated hFOB 1.19 and OS cells (143B, SaoS2, MG63, U2OS, and HOS) using RT-qPCR. The mRNA expression level of PKMYT1 in 143B (d), HOS (e), and U2OS (f) after treatment with different concentrations of DDP for 24 h. g The difference in PKMYT1 mRNA expression levels between the DDP-resistant cell line U2OS/DDP and the parental cell line U2OS. h–i The expression of PKMYT1 in 143B, HOS, and U2OS cells after treatment with varying concentrations of DDP for 24 h, as well as the difference in PKMYT1 protein expression between the DDP-resistant cell line U2OS/DDP and the parental cell line U2OS, was assessed using Western blot analysis. j IHC staining of PKMYT1 was performed on 20 osteosarcoma (OS) tissue samples, including both post-chemotherapy and pre-chemotherapy tissues. Representative images are shown, and quantification was analyzed using a t test. Data shown are mean ± SD Error bars, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001