Fig. 7

Lactate enhances the expression of Arg1 through the upregulation of H3K18la. a Assessment of relative intracellular lactate levels of VSMCs at different time points under hypoxia (n = 3 independent experiments). b Western blot analysis of the expressions of Arg1, H3K18la, H4K5la, and LDHA at different time points under hypoxia (n = 3 independent experiments). c Immunofluorescence detection of the effect of hypoxia on H3K18la in VSMCs (bar = 5 μm) (n = 3 independent experiments). d The effect of lactate stimulation at different concentrations on Arg1 expression (n = 3 independent experiments). e The effect of lactate stimulation at different concentrations on H3K18la expression (n = 3 independent experiments). f H3K18la occupancy analysis of VSMCs by ChIP–qPCR (n = 3 independent experiments). g The relative intracellular lactate levels of VSMCs were treated with different concentrations of Oxamate after hypoxia for 4 hours (n = 3 independent experiments). h Immunofluorescence on the effect of different concentrations of Oxamate on H3K18la (bar = 5 μm) (n = 3 independent experiments). i The LDHA, H3K18la and Arg1 expressions of VSMCs treated with different concentrations of Oxamate after hypoxia for 4 hours (n = 3 independent experiments). j H3K18la occupancy analysis of hypoxic VSMCs after being treated with Oxamate (n = 3 independent experiments). a: p < 0.05, as compared with the 0 h group; b: p < 0.05, as compared with the 0 mM group