Fig. 1

CTC acquire an ENPP1+ signature displaying genome integrity and stemness traits. a Schematic of the isolation of engrafting “CTC-in” at the RTB after the intracardiac inoculation in previously orthotopically tumor resected mice (generated by BioRender). b Selected hierarchical functional GO categories based on their biological relevance related to the observed phenotype, obtained by transcriptomic analysis of gene signatures from two different independently isolated CTC-in cells derived from each ANV5 (CTC_A_B5coh) and 4T1 (CTC_T_B5coh) cell lines, compared to their respective parental cells using RNA-seq. Number of coherent genes with B > 5 appears in parenthesis for each cell line. c Hierarchical cluster of transcriptomic upregulated genes related to “Response to radiation” (Left panel) and Stemness (Right panel) GO categories obtained in two different independently isolated CTC-in cells derived from each ANV5 and 4T1 parental cell lines. d Validation by RT-qPCR of commonly regulated genes expressed in different CTC-in derivatives from ANV5 (700, 803, and 054) and 4T1 (1589, 1592, 1593 and 1600) isolated at the RTB compared to their respective parental ANV5 and 4T1 cell lines