Fig. 2

Heatmaps showing differentially-expressed proteins (DEPs) per tissue. DEPs were determined using a |log2(FC| > 1 and q < 0.05, comparing different Hit-Time groups to SHAM. In the heatmaps, red indicates high expression levels while blue represents low expression levels. Prior to clustering, the protein expression was centered and scaled. a Hierarchical clustering was conducted using Euclidean distance and average linkage to cluster the samples. Proteins were clustered using pearson correlation and average. More details about DEPs can be found in Supplementary Table 2. b Venn diagrams and heatmaps displaying the overlapped proteins that were deregulated in the hippocampus and cortex. Unique proteins in blue, red, or green, commonly altered proteins in the intersection of red, blue, and green. The Heat maps are based on the hierarchical clustering analysis corresponding to the detected proteins within the following conditions: sham, 1 hit, and 3 hits after 48 h or1 week post-injury taking the ipsilateral and contralateral hippocampus, and cortex regions (HI-I: Hippocampus 1 hit, HI-II: Hippocampus 3 hits, HC- I: Cortex 1 hit, HC-II: Hippocampus 3 hits). Distinct clusters are highlighted and assigned numbers from 1 to 7. Red corresponds to the upregulated proteins whereas green refers to the downregulated proteins. More details about the clusters can be found in Supplementary Table 3. c LDA plot and its classification report. Matrix confusion and classification report of LGBM model. Matrix confusion and classification report of the best and optimal model Ridge