Fig. 5 | Signal Transduction and Targeted Therapy

Fig. 5

From: Transferrin receptor 1 nuclear translocation facilitates tumor progression via p53-mediated chromatin interactions and genome-wide alterations

Fig. 5

Cellular function demonstrates the role of nuclear TfR1 in tumor progression in response to DNA damage. a Assessment of apoptosis in HCT-116 shSCR or shTFRC cells after 50 μM CDDP treatment for 24 h. b Quantification of the percentage of apoptotic cells (a) (n = 3). c Assessment of apoptosis in response to 50 μM CDDP stimulation for 24 h with or without pretreatment with 500 nM BFA for 6 h. d Quantification of the percentage of apoptotic cells in (c) (n = 3). e Representative images of the alkaline comet assay after 1 μM CDDP treatment for 6 h with or without pretreatment with 500 nM BFA for 6 h. Scale bar = 100 μm. f Quantification of the percentage of DNA in the comet tail (% Tail DNA) of (e) (n = 50). The data were presented as the means ± SEMs from at least three independent experiments in (b, d, and f). The P-value was determined using two-way ANOVA analysis in (b, d, and f). ****P < 0.0001

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