Fig. 6

Influence of nuclear TfR1 on tumor progression. a Representative 2 cases of IHC images (×2, ×10, and ×20 magnifications) from the CRC tissue microarray (n = 151). Scale bar = 500 μm (magnification ×2); scale bar = 100 μm (magnification ×10); scale bar = 50 μm (magnification ×20). b–d Correlations between the nuclear TfR1 level score and WHO grade (b), AJCC stage (c), and lymph node involvement (d) in CRC samples. e Representative 2 cases of IHC images (×2, ×10, and ×20 magnifications) of TfR1 and XPC staining in the CRC tissue microarray. Scale bar = 500 μm (magnification ×2); scale bar = 100 μm (magnification ×10); scale bar = 50 μm (magnification ×20). f Chi-square test of the correlation between the levels of nuclear TfR1 and XPC determined via IHC staining. g Representative images from RNA-FISH analysis showing XPC mRNA levels in HCT-116 shSCR- or shTFRC-xenografted tumor tissues (n = 5). CDDP (1 mg/kg) was intratumorally injected into the tumors 24 h before harvest. Nuclei were stained with DAPI. Scale bar = 10 µm. h Quantification of the relative fluorescence intensity of RNA-FISH images. The data were presented as the means ± SEMs of at least three independent experiments in (h). P-values were determined via one-way ANOVA in (b), via unpaired two-tailed Student’s t-tests in (c, d, and h), and via chi-square tests and Fisher’s exact tests in (f). ***P < 0.001