Table 1 Data collection and refinement statistics (molecular replacement)
Nipah Virus attachment (G) glycoprotein in complex with neutralizing antibody 14F8 | Hendra Virus attachment (G) glycoprotein mutant S586N | Hendra Virus attachment (G) glycoprotein mutant S586N in complex with neutralizing antibody 14F8 | |
|---|---|---|---|
Data collection | |||
Space group | P 41 21 2 | P 1 21 1 | C 2 2 21 |
Cell dimensions | |||
a, b, c (Å) | 125.96 125.96 317.26 | 110.84 257.01193.52 | 110.84 257.01193.52 |
α, β, γ (°) | 90.00 90.00 90.00 | 90.00 97.69 90.00 | 90.00 90.00 90.00 |
Resolution (Å) | 36.45–2.79 | 41.59–3.30 | 37.79–3.22 |
Rsym or Rmerge | 0.10 | 0.148 | 0.214 |
I/σI | 1.75 (at 2.81 Å) | 3.08 (at 3.32 Å) | 1.53 (at 3.25 Å) |
Completeness (%) | 98.9 | 98.5 | 98.9 |
Redundancy | 13.2 (10.4) | 6.2 (5.7) | 5.1 (4.6) |
Refinement | |||
Resolution (Å) | 36.23–2.80 | 41.59–3.3 | 37.06–3.22 |
No. reflections | 63450 | 19266 | 44342 |
Rwork/Rfree | 21.93/24.35 | 23.2/28.13 | 19.45/22.74 |
No. atoms | 13483 | 6453 | 13414 |
Protein | 13096 | 6354 | 13086 |
Ligand/ion | 0 | 84 | 0 |
Water | 117 | 236 | 328 |
B-factors | 62.49 | 101.44 | 67.55 |
Protein | 62.59 | 101.28 | 66.63 |
Ligand/ion | 118.01 | ||
R.m.s. deviations | |||
Bond lengths (Å) | 0.004 | 0.003 | 0.004 |
Bond angles (°) | 0.83 | 0.70 | 0.78 |
Ramachandran favored (%) | 93.81 | 93.92 | 96.84 |
Ramachandran allowed (%) | 6.19 | 6.08 | 3.16 |
Ramachandran outliers (%) | 0 | 0 | 0 |
