Fig. 2
Soma, axonal and dendritic alterations of PCs in Slc38a6-/- mice. a Quantification of the PC linear density of Slc38a6+/+ and Slc38a6-/- mice at 2, 4, 6, 9, and 12.5 months (n = 6 mice per group). b Representative H&E-stained sagittal cerebellar sections of Slc38a6+/+ and Slc38a6-/- mice at 12.5 months. c Golgi-stained PC dendrites in 2-, 4-, 6-, 9-, and 12.5-month-old Slc38a6+/+ and Slc38a6-/- mice. d‒h Age-matched comparisons of dendrites of Slc38a6+/+ and Slc38a6-/- mice at 2 (d), 4 (e), 6 (f), 9 (g), and 12.5 (h) months were performed via Sholl analysis (n = 20 stained PCs from 4 mice for each group). i Representative PC axons stained with calbindin-D28K. Abnormal alterations of PC axons in Slc38a6-/- mice at 12.5 months. Left, normal PC axons in Slc38a6+/+ mice at 12.5 months; middle, two torpedoes (arrowheads) and branched axons (arrows); right, thickened axonal profiles (arrows). j Comparison of torpedoes between Slc38a6+/+ and Slc38a6-/- mice at 12.5 months (n = 5 mice per group). k Comparison of thickened axonal profiles between Slc38a6+/+ and Slc38a6-/- mice at 12.5 months (n = 5 mice per group). The data are presented as the means ± SEMs. Statistical tests: two-way ANOVA followed by Sidak’s multiple comparison tests (a), (d‒h) and the Mann‒Whitney test (j), (k). Scale bars: 50 μm (b); 20 μm (c); 50 μm (i)
