Fig. 4
Abnormal functions of PCs in Slc38a6-/- mice and Slc38a6PC-/- mice. a Representative APs of Slc38a6+/+ and Slc38a6-/- PCs evoked by current steps of 200 pA and 600 pA. b Number of APs induced by the current steps in Slc38a6+/+ (n = 19, black) and Slc38a6-/- (n = 15, blue) PCs. c–e Summary of the threshold (c), amplitude (d) and half-width (e) of the APs in Slc38a6+/+ and Slc38a6-/- PCs. f Representative sIPSC traces in Slc38a6+/+ and Slc38a6-/- mice. NBQX is an AMPA receptor antagonist. D-APV is an NMDA receptor antagonist. g Cumulative distribution of interevent intervals and summary graphs of sIPSC frequency (inset) in Slc38a6+/+ (n = 17, black) and Slc38a6-/- (n = 16, blue) PCs. h Same as panel (g) but for sIPSC amplitude. i Representative APs of control and Slc38a6PC-/- PCs evoked by current steps of 200 pA and 600 pA. j Number of APs induced by the current steps in control (n = 17, black) and Slc38a6PC-/- (n = 20, blue) PCs. k‒m Summary of the threshold (k), amplitude (l) and half-width (m) of the APs in control and Slc38a6PC-/- PCs. n Representative sIPSC traces in control and Slc38a6PC-/- mice. NBQX is an AMPA receptor antagonist. D-APV is an NMDA receptor antagonist. o Cumulative distribution of interevent intervals and summary graphs of sIPSC frequency (inset) in control (n = 21, black) and Slc38a6PC-/- (n = 17, blue) PCs. p Same as panel (o) but for sIPSC amplitude. Note that sIPSC frequency was increased in Slc38a6PC-/- PCs and that there was no change in sIPSC amplitude. The data are presented as the means ± SEMs. Statistical tests: two-way ANOVA followed by Sidak’s multiple comparison tests (b), (j); two-tailed unpaired Student’s t test (c‒e), (k‒m); Kolmogorov‒Smirnov test; Inner of (g), (h), (o), (p)
