Fig. 2 | Signal Transduction and Targeted Therapy

Fig. 2

From: Ninjurin-1 mediates cell lysis and detrimental inflammation of PANoptosis during influenza A virus infection

Fig. 2

Oligomerization of NINJ1 synchronizes with PANoptosis during IAV infection. a, b Immunoblots of indicated proteins in BMDMs at indicated h.p.i. (hours post infection), BS3-crosslinked (a) or non-crosslinked (b). FL, full length; c-, cleaved; Lys., lysates; Sup., supernatants. c–f Representative images (c) and quantification (d) of PI+ cells, cell viability (e), and LDH release (f) in BMDMs treated as panel (a, b). Scale bars, 100 μm. g Silver staining of supernatants from (b). h, i Concentrations of IL-1β (h) and TNF-α (i) in supernatants from (f). Data are representative of three independent experiments and are presented as mean ± SD. Two-way ANOVA was used. *p < 0.05; ****p < 0.0001

Back to article page