Fig. 4 | Signal Transduction and Targeted Therapy

Fig. 4

From: Ninjurin-1 mediates cell lysis and detrimental inflammation of PANoptosis during influenza A virus infection

Fig. 4

NINJ1-mediated cell lysis depends on ZBP1. a, b Representative images (a) and quantification (b) of PI+ cells of WT, Zbp1-/- and Ifnar1-/- BMDMs at 16 hpi. Scale bars, 100 μm. c, g Immunoblots of the indicated proteins in the BMDMs (genotypes as above) at 16 hpi, BS3-crosslinked (g) or non-crosslinked (c). d Cell viability of WT, Ninj1-/-, Zbp1-/- and Ifnar1-/- BMDMs at 16 hpi. e LDH release in WT, Zbp1-/-, and Ifnar1-/- BMDMs with/without glycine (10 mM) at 16 hpi. f Silver staining of supernatants from (c). h, i LDH (h) and IL-1β (i) release in WT, Ninj1-/-, and Zbp1-/- BMDMs at 16 hpi. Data are representative of three independent experiments and are presented as mean ± SD. Kruskal‒Wallis test (d) or two-way ANOVA (b, e, h, and i) was applied. ns, not significant; **p < 0.01; ***p < 0.001; and ****p < 0.0001

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