Fig. 5 | Signal Transduction and Targeted Therapy

Fig. 5

From: Ninjurin-1 mediates cell lysis and detrimental inflammation of PANoptosis during influenza A virus infection

Fig. 5

Activation of any PANoptosis pathway induces NINJ1 oligomerization during IAV infection. a, d Immunoblots of NINJ1 in BMDMs of indicated genotypes at 16 hpi, BS3-crosslinked. b Viability of BMDMs of indicated genotypes at 16 hpi. c LDH release in BMDMs of indicated genotypes at 16 hpi, with/without glycine (10 mM). e, g Immunoblots of indicated proteins in WT, Gsdmd-/-Gsdme-/-, and Gsdmd-/-Gsdme-/-Mlkl-/- BMDMs at 16 hpi, treated with DMSO, Z-IETD-FMK (25 μM), or Z-VAD-FMK (25 μM), BS3-crosslinked (g) or non-crosslinked (e). f Representative images of PI+ cells from panel (e). Scale bars, 100 μm. See also Supplementary Fig. 5f. h, i Cell viability (h) and LDH release (i) in WT, Gsdmd-/-Gsdme-/-, and Gsdmd-/-Gsdme-/-Mlkl-/- BMDMs at 16 hpi, treated with DMSO, Z-IETD-FMK, Z-VAD-FMK, or Z-VAD-FMK plus glycine. Data are representative of three independent experiments and are presented as mean ± SD. Kruskal‒Wallis test (b) or two-way ANOVA (c, h, and i) was applied. ns, not significant; **p < 0.01; and ****p < 0.0001

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