Fig. 6

NINJ1 drives IAV-induced lung pathology and hyperinflammation. a−c Survival curves of (a) Ninj1^-/- mice and their littermates; (b) Zbp1^-/- and WT mice; (c) Gsdmd^-/-, Gsdme^-/-, Mlkl^-/-, and WT mice. d, e H&E staining (d) and pathological scores (e) of the same lung lobe of WT and Ninj1^-/- mice at 5 dpi. The right panel in (d) shows magnified images of the insects. Scale bars, 2 mm and 0.2 mm (insects). f Viral titers in lungs of WT, Ninj1^-/- and Zbp1^-/- mice at 5 dpi. g Cellular pellets in BALF of WT and Ninj1^-/- mice at 8 dpi after centrifugation. h−l, o Concentrations of total proteins (h) and indicated cytokines (i−l, o) in BALF of WT and Ninj1^-/- mice at 3, 6, and 8 dpi. m, n Frequencies of CD45⁺ cells (m) and Ly6C⁺ monocytes (n) among total live cells in BALF of WT and Ninj1^-/- mice at 3 and 6 dpi. Gating strategies are shown in Supplementary Fig. 9a. Mice were infected with LD₁₀₀ (a, c) or LD₅₀ (b, d–o) of IAV. Data are representative of at least two independent experiments and are presented as mean ± SD. Two-way ANOVA (h‒o), one-way ANOVA (f), or Student’s t-test (e) was used. Survival curves were analyzed by the log-rank test (a−c)