Fig. 1 | Signal Transduction and Targeted Therapy

Fig. 1

From: Retinol Binding Protein 4 reactivates latent HIV-1 by triggering canonical NF-κB, JAK/STAT5 and JNK signalling

Fig. 1

Identification of RBP4 as an enhancer of latent HIV. a (Left panel) Activation of latent HIV-1 eGFP reporter proviruses in J-Lat 11.1 cells treated with peptide fractions of a hemofiltrate (HF)-derived library. The color of the bar indicates to which pool of the peptide library the fraction belongs. Mock indicates the absence of peptide fractions; PMA and TNFα were used as positive controls. Fractions highlighted by the upper bar were used for further purification. (Right panel) MALDI-TOF spectrum of RBP4 shows a dominant signal of m/z 20,960.75, which closely matches the theoretical m/z value of the RPB4 (1-182, 20,959.42 Da) truncated variant lacking the C-terminal leucin. b Example for activation of latent HIV-1 in J-Lat 11.1 cells by a single HF-derived fraction compared to the PMA and TNFα controls, determined by flow cytometry. c The levels of RBP4 in subfractions of the pool 8 HF fraction 31 detected by SDS-PAGE after purification (lower panel) correlate with the percentages of cells showing reactivation of HIV-1 eGFP reporter proviruses

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