Fig. 3 | Signal Transduction and Targeted Therapy

Fig. 3

From: Single-cell RNA-seq reveals cell type-specific molecular and genetic associations with primary open-angle glaucoma

Fig. 3

Extensive and cell type-specific pathway dysregulation in circulating immune cells from POAG patients. a Bar plot showing the number of significantly upregulated (red) and downregulated (blue) KEGG and Reactome pathways in each immune cell subtype between POAG patients and controls. b Heatmap summarizing the overall pathway activity score (−log10 adjusted P value, up- or down-) for each major immune cell subtype in POAG patients versus controls by Partition-based graph abstraction (PAGA) analysis. c Heatmap of significantly upregulated KEGG pathways (rows) across immune cell subtypes (columns) in POAG patients versus controls. The color intensity reflects the significance and direction of change. d Heatmap of significantly downregulated KEGG pathways (rows) across immune cell subtypes (columns) in POAG patients versus controls. e PAGA network showing the relationships and pathway activity changes among immune cell subtypes in POAG patients versus controls. Node color indicates up- (red) or downregulation (green) of pathway activity. f, g Violin plots showing the relative expression levels of the TNF (f) and IFNG (g) genes in PBMCs from POAG patients and controls. P values are indicated. h Combined pathway heatmap (natural style) displaying the relative activity of selected immune, metabolic, and neurodegenerative pathways (rows, grouped and color-coded by pathway type) across immune cell subtypes (columns) in POAG patients versus controls. The color scale represents normalized pathway activity scores

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