Fig. 3 | Signal Transduction and Targeted Therapy

Fig. 3

From: Targeting fused in sarcoma (FUS): a novel antisense strategy for treating idiopathic pulmonary fibrosis

Fig. 3

FUS binds to profibrotic RNAs in IPF fibroblasts. a Tabular form of samples from HD or IPF fibroblasts used for the experiment involving cross-linking immunoprecipitation and RNA sequencing (CLIP-Seq). b Scheme representing the workflow of CLIP-Seq using an anti-FUS antibody for IP. c Representative western blot analysis of the CLIP-Seq samples before RNA extraction showing efficient pulldown of the FUS protein. d Principal component analysis (PCA) for the multiple groups involved is shown. e A Z-score-normalized heatmap of the top 50 DEGs enriched with FUS protein in IPF versus HD fibroblasts is shown. f Volcano plot highlighting the top 4 genes, COL5A1, TGFB1, COL4A2, and IL11, which are profibrotic genes out of the 1937 upregulated genes/genes bound to FUS in the FUS-IP of IPF fibroblasts. g Twenty profibrotic genes that were upregulated/FUS interactors among the top 50 DEGs are listed here, along with the significance (FDR) and log2FC for each gene. h Gene set enrichment of the top 50 sets, as analyzed by KOBAS for Reactome, is shown. CLIP-Seq was performed on HD and IPF fibroblasts (n = 4 each)

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