Fig. 4 | Signal Transduction and Targeted Therapy

Fig. 4

From: Targeting fused in sarcoma (FUS): a novel antisense strategy for treating idiopathic pulmonary fibrosis

Fig. 4

Silencing FUS regulates genes involved in profibrotic signaling in IPF fibroblasts. a IPF fibroblasts were either treated with a nontargeting sequence of antisense oligonucleotide (ASO; Scr) or ASO for FUS (ION363; 15 µg) for 72 h. Representative western blots and quantifications from cell lysates of four IPF patient fibroblasts showing a decrease in the protein level of FUS upon ION363 treatment. b PCA showing well-separated data sets from these two groups. c A Z-score heatmap plot of the top 50 DEGs is shown. d Gene enrichment of the top 50 data sets identified via KEGG pathway analysis is shown, with cell cycle genes being the genes most significantly enriched upon ION363 treatment in IPF fibroblasts. e Gene enrichment analysis of the top 50 sets via kobas for the PANTHER database, showing genes of the extracellular matrix to be differentially regulated. Blots and analyses were performed on fibroblasts derived from n = 4 IPF patients/group

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