Fig. 5
ION363 regulates multiple profibrotic, FUS-interacting genes in IPF fibroblasts. The data set from CLIP-Seq (for FUS, Fig. 1) was compared with the data set obtained from ION363-treated IPF fibroblasts (Fig. 5). a Venn diagram of the comparison: With the indicated FDR and log2FC cutoff values, approximately 82 genes that were identified as FUS interactors were downregulated by ION363, and approximately 48 FUS interactor genes were upregulated by ION363. b A Venn diagram with a more stringent analysis of our data set is shown, where the stringency was set to FDR < 0 and log2FC > 2.0 for the CLIP-Seq data. In IPF fibroblasts, 50 FUS-interacting genes were downregulated, and approximately 16 were upregulated upon ION363 treatment. c A Z-score heatmap plot for the 50 FUS-interacting genes whose expression was downregulated by ION363 is shown. d STRING analysis of these 50 genes shows the involvement of these genes in known and unknown predicted interactions. e Analysis of these 50 genes revealed that these downregulated genes were involved in tissue development, cell communication, lung fibrosis, and wound healing. f The 16 FUS-interacting genes that are upregulated by ION363 treatment are shown
