Fig. 8 | Signal Transduction and Targeted Therapy

Fig. 8

From: Targeting fused in sarcoma (FUS): a novel antisense strategy for treating idiopathic pulmonary fibrosis

Fig. 8

FUS silencing enhances alveolar regeneration and epithelial differentiation in IPF-derived alveolospheres. a Alveolospheres treated with Scr-ASO (Scr) or ION363 were cultured until day 21 and stained with LysoTracker Red and Hoechst (nuclei), followed by live imaging at 4× in an EVOS M7000. Stitched images with or without TD (transmitted light/brightfield) were saved via Celleste 6.0. Scale bar = 2 mm. b The number of alveolospheres formed per plated cell number in each treatment was counted and quantified, or c each alveolosphere was measured via Celleste 6.0 software. P-value summary: *P < 0.05, ***P < 0.001. d Representative images of one 3D live alveolosphere stained with LysoTracker/Hoechst and imaged via an EVOS M7000 at 20×. e Representative images of one fixed alveolosphere from each treatment group that was stained with HTII-280 (green), AQP5 (red), or Hoechst (blue) and imaged via an Olympus FV3000 microscope. Scale bar = 20 µm. Representative images and analyses of alveolospheres from n = 3 IPF patients are shown

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