Abstract
Study design
Pilot study.
Objectives
Single-nucleotide polymorphisms (SNPs) in TRPA1 gene are related to the etiology of chronic pain. The study is a pilot study with the primary objective of analyzing these SNPs in Spanish patients with chronic and complete spinal cord injury (SCI) and neuropathic pain (NPP).
Setting
Asepeyo Hospital Department of Chronic and Complete SCI.
Methods
Twelve patients with chronic and complete SCI and NPP, and 12 patients with chronic and complete SCI with no pain were reviewed. International Spinal Cord Injury Pain Classification (LANSS) and visual analog score (VAS) were chosen to classify pain syndrome. SNPs were identified by melting analysis after DNA amplification with real-time fluorescence PCR.
Results
There were differences in rs11988795 variant: GG homozygous (p = 0.01) and G allele (p = 0.001) were more frequent in SCI patients with no pain. There were differences in rs13255063 variant: TT homozygous were prevalent (p = 0.03) in patients with NPP.
Conclusions
Until now this is the first study to show a description of TRPA1 SNPs in Spanish patients with chronic and complete SCI and NPP. These results suggest that GG genotype in rs11988795 variant and G allele could be protective factors against NPP. TT genotype in rs13255063 variant could be a risk factor for NPP. Neuropathic pain after spinal cord injuries may have genetic contributions.
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Acknowledgements
Spanish Abertis Foundation: This study was funded by Abertis Foundation of Spain. Abertis is the international market leader in the management of toll roads, managing over 8600 km of high capacity and quality roads around the world. Its ongoing internationalization process has led Abertis to be present in 14 countries in Europe, the America, and Asia, and this has enabled it to diversify its geographic risk and better adapt to global business cycles. Abertis is the first national toll road operator in countries such Spain and Chile, and has a highlighted presence in France, Brazil, Italy, and Puerto Rico. The company also has a stake in more than 700 km through different concessionaires in United Kingdom, Argentina, and Colombia. Over 70% of the Group’s revenue is generated outside Spain. Especially important in this respect are France, Brazil, and Chile. Abertis is listed on the Spanish Stock Exchange and is on the IBEX 35 and the international FTS Eurofirst 300 and Standard & Poor’ Europe 350 indexes. The Abertis Foundation was established in 1999 as a non-profit organization, with the objective of responding to the impact that the Abertis Group’s economic activity has in the different territories and countries where the Group is present. The Foundation has always prioritized actions related to road safety, the environment, and social action, in line with the Abertis Corporate Social Responsibility Strategic Plan.
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S.V.V.R. is first author and the principal researcher. All authors have read and approved the final submitted manuscript.
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Vidal Rodriguez, S., Castillo Aguilar, I., Cuesta Villa, L. et al. TRPA1 polymorphisms in chronic and complete spinal cord injury patients with neuropathic pain: a pilot study. Spinal Cord Ser Cases 3, 17089 (2017). https://doi.org/10.1038/s41394-017-0004-0
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DOI: https://doi.org/10.1038/s41394-017-0004-0
