Table 1 Summary of the analyses of the autoradiographs

	Sulfidic pulse		Oxic pulse		24-h oxic + oxic pulse		Sulfidic pulse-chase		Wilcoxon–Mann–Whitney test
	AGD	RGD	AGD	RGD	AGD	RGD	AGD	RGD	sp vs op	sp vs spc
Microzooid symbiont	27.66	107.78 **	3.63	112.88 **	0.12	–	21.39	103.36	nd	nd
	(16.97–28.93)	(107.53–110.50)	(3.29–3.67)	(102.94–117.83)	(0.12–0.13)	–	(16.06–27.34)	(101.36–103.83)
Stalk symbiont	9.20	51.75	2.03	68.30	0.02	–	16.63	80.97	nd	nd
	(8.17–10.65)	(31.61–69.82)	(2.00–3.58)	(61.81–68.76)	(0.01–0.08)	–	(14.22–21.32)	(79.31–90.69)
Total symbiont	23.06	100.00	2.97	100.00	0.09	–	19.79	100.00	nd	nd
	(15.79–25.67)	(100–100)	(2.91–3.63)	(100–100)	(0.08–0.11)	-	(15.96–26.33)	(100–100)
Microzooid host	10.44	44.36	1.05	32.65	0.07	–	12.65	63.89	nd	*
	(4.69–11.39)	(35.62–45.25)	(0.71–1.18)	(32.53–35.56)	(0.06–0.10)	–	(9.36–21.37)	(63.12–69.60)
Stalk host	4.17	19.38	0.63	21.61	0.03	–	9.13	50.53	nd	**
	(0.46–5.60)	(2.89–21.81)	(0.43–1.25)	(14.51–24.57)	(0.03–0.03)	–	(8.79–11.56)	(44.40–67.85)
Total host	4.56	24.90	0.68	20.00	0.09	–	12.92	65.26	nd	*******
	(2.31–6.39)	(9.99–33.75)	(0.59–1.10)	(19.04–30.29)	(0.04–0.11)	–	(10.73–18.38)	(61.59–69.8)

For each incubation, the medians of the actual grain density (AGD) and relative grain density (RGD) of five replicates are shown together with the interquartile range of the data (Q25–Q75). The RGDs are the grain counts, which are set as a proportion of the overall symbiont grain count. All comparisons were then performed on the RGDs. Within a particular treatment, comparisons of the symbionts covering the microzooid and stalk areas as well as the respective host areas were performed using Wilcoxon–Mann–Whitney significance testing. This test was also performed to compare the sulfidic pulse and the oxic pulse (sp vs op), as well as the sulfidic pulse and the sulfidic pulse chase incubations (sp vs spc). Bonferroni correction was then applied
nd not different
*p < 0.05; **p < 0.01; ***p < 0.001

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