Fig. 5

Detection of Formosa strain B laminarin PULs in the Helgoland spring bloom metagenome and metaproteome in 2009 [17]. a Synteny between the laminarin PULs of Formosa sp. Hel1_33_131 and partial PUL sequences in the metagenomes from 2009/04/07. The sequence comparisons were performed with Bl2seq (BLASTn, E value 1e-5). Sequence similarities are depicted by red hues for direct comparisons. Darker colors correspond to higher identities. Gene locus tags are subsequent numbers within PULs and are indicated in the figure for most of the genes (for visibility’s sake, gene names of very small genes were omitted). b Heatmap of the relative abundance of Formosa strain B proteins (displayed as normalized spectral abundance factor values, NSAF*1000) detected in the metaproteome from 07 April 2009. Displayed are selected proteins, which likely play a role in polysaccharide or protein utilization. A highly abundant ribosomal protein of Formosa strain B (rib. protein S1, lower right) is also displayed as a reference to illustrate the high abundance of polysaccharide utilization-specific proteins during the bloom condition. Gene locus tag numbers are given in parenthesis. GH, glycoside hydrolase; PKD, PKD-domain containing protein; SusD, SusD-family protein; TBDR, TonB-dependent receptor; HP, hypothetical protein; MFS, major facilitator superfamily; ExbD, subunit of the Ton system for energy transduction; MotB, motor rotation protein; Gld, gliding motility proteins; PDHE, pyruvate dehydrogenase E1 component; KDPG, 2-dehydro-3-deoxyphosphogluconate aldolase; GPI, glucose-6-phosphate isomerase; PKFM, 6-phosphofructokinase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; P-II, nitrogen regulatory protein P-II; PSAT, phosphoserine aminotransferase; SHMT, serine hydroxymethyltransferase