Fig. 2 | The ISME Journal

Fig. 2

From: Functional metagenomics reveals abundant polysaccharide-degrading gene clusters and cellobiose utilization pathways within gut microbiota of a wood-feeding higher termite

Fig. 2

Biochemical characterization of the four xylanases on the fosmid contig00057. a Schematic organization of lignocellulase genes on contig00057. b 12% SDS–PAGE analysis of the four purified xylanases. Lanes: M, protein molecular weight marker; lane 1–4: purified recombinant proteins for Xyl-ORF7, Xyl-ORF19, Xyl-ORF20 and Xyl-ORF21, with molecular weights of 45.1, 51.3, 42.3 and 53.9 kDa, respectively. c TLC analysis. Marker (M), xylose (X1), xylobiose (X2), xylotriose (X3), xylotetraose (X4), xylopentose (X5), and xylohextose (X6). a hydrolytic products of 10 min of the four xylanases against birch wood xylan; b hydrolytic products of 12 h of the four xylanases against birch wood xylan. d pH and temperature profile. (i) Temperature range of Xyl-ORF7, Xyl-ORF19, Xyl-ORF20 and Xyl-ORF21 were assayed between 30 and 70 °C. (ii) Thermostability were measured by dectecting the residual activity after preincubation at 50 °C for 5 min, 10 min, 15 min, and 30 min respectively. (iii) pH range were assayed between pH 4.5 and 10. (iv) pH stability were examined after preincubation in buffers ranging from pH 4.5 to 10 at 4 °C for 5 days. All activity assays were obtained from triplicate experiments

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