Fig. 4

Spatial and environmental relationships of four Microcoleus species. a Location of genomes from different Microcoleus species, which are indicated with a different shape and color (genome locations were jittered to prevent overlap of multiple genomes recovered at a single site). Site numbers are indicated in black (see Fig. 1). b Pairwise comparisons of distances between collection sites along the river network and average nucleotide identity (ANI) percentage between all genomes. Comparisons between genomes that came from the same Microcoleus species (within species comparisons) are identified by colors and shapes, all comparisons between genomes from different Microcoleus species are indicated by a black star. The black line shows GAM predictions for all comparisons (within and between species) surrounded by 95% confidence intervals in light gray. c Dissolved nutrient concentrations and Microcoleus species distributions at different sampling sites. Each number on the figure indicates what Microcoleus species were present at a site, and colors correspond to the Microcoleus species in panel a. If more than one species were present at a site, the species numbers are separate by commas. The x-axis represents the upstream watershed area at each site. d Generalized dissimilarity model (GDM) I-spline partial effects of environmental variables on ANI. The maximum height of each I-spline curve is proportional to the amount of ANI variation associated with that variable. The shape and slope of the curve shows the changing relationship between ANI values and environmental variables along the range of values for each variable