Fig. 5

Features of metagenomes reflecting possible adaptations to hyperalkaline conditions. Plot depicting the distribution of estimated genome sizes (in Mbp) of metagenome-assembled genomes (MAGs) (a). MAGs are represented by circles ordered from left to right by increasing pH of the waters that hosted these communities. The size of each circle corresponds to the estimated relative abundance of each MAG, as indicated in the legend on the bottom right of the figure. Plot of median estimated genome sizes (in Mbp) of MAGs as a function of pH (b). Each point depicts the median estimated MAG size (in Mbp) for each well. Error bars depict the standard deviation of the estimated sizes of all MAGs with at least 40% estimated completeness within each metagenome. Plot of the weighted average oxidation state of carbon (Z_c) in inferred proteomes of eight metagenomes as a function of pH (c). Each point represents the weighted Z_c value calculated for each metagenome based upon the Z_c per amino acid in the inferred protein sequences encoded by each metagenome. Z_c values were then normalized to protein length and read coverage of the gene encoding each specified protein and finally to the number of base pairs sequenced per metagenome