Fig. 4

Evolutionary history of the group 1h [NiFe]-hydrogenase and type I carbon monoxide dehydrogenase. Phylogenetic trees showing the distribution and evolutionary history of the catalytic (large) subunits of the group 1h [NiFe]-hydrogenase (hhyL; a) and type I carbon monoxide dehydrogenase (coxL; b) in the phylum Chloroflexi. Chloroflexi sequences (labelled by class) are shown in bold against reference sequences (labelled by phylum). Trees were constructed using amino acid sequences through the maximum-likelihood method (gaps treated with partial deletion) and were bootstrapped with 100 replicates. The trees were respectively rooted with group 1g [NiFe]-hydrogenase sequences (WP_011761956.1, WP_048100713.1) and type II carbon monoxide dehydrogenase sequences (WP_011388721.1, WP_012893108.1). The distribution of other respiratory uptake hydrogenases within genomes and metagenome-assembled genomes (MAGs) in the phylum Chloroflexi is shown in Figure S4. The distribution of type I carbon monoxide dehydrogenases within metagenome-assembled genomes (MAGs) in the phylum Chloroflexi is shown in Figure S5