Fig. 1

CA activity of purified recombinant LCIP63. a CA activity with and without treatment with 5 mM EDTA or EDTA plus 10 mM of either Zn²⁺, Mn²⁺, Ca²⁺, Co²⁺, or Mg²⁺. n.s, not significant; ***p ≤ 0.001. Error bars show mean ± SD, n = 3–19. ‘a’ indicates values compared to the EDTA-treated sample. b CA activity in LCIP63 treated with 20 µM acetazolamide (AZA) versus control; *p ≤ 0.05. c Esterase activity of LCIP63 expressed as the production of p-nitrophenol (µmol l⁻¹). The arrow represents the time when the substrate p-nitrophenyl acetate was added to the reaction mixture. d Phylogenetic tree constructed from sequences of the different CA subclasses; numbers in parentheses are the mean percent identity with LCIP63. Pfal, Plasmodium falciparum; Prei, Plasmodium reichenowi; Plasmo, Plasmodium sp.; Pgab, Plasmodium gaboni; Pt, Phaeodactylum tricornutum; Fs, Fistulifera solaris; Fc, Fragilariopsis cylindrus; Tp, Thalassiosira pseudonana; Ehux, Emiliania huxleyi; Lpol, Lingulodinium polyedra; To, Thalassiosira oceanica; Esil, Ectocarpus siliculosus; Blasto, Blastocystis sp.; Pinf, Phytophtora infestans; Phal, Plasmopara halstedii; Sjap, Saccharina japonica; Tw, Thalassiosira weissflogii; Pm, Pseudo-nitzschia multiseries