Fig. 3
From: A new widespread subclass of carbonic anhydrase in marine phytoplankton
LCIP63 overexpression and effect on photosynthesis in T. pseudonana. a Western blot against LCIP63 (upper) and SDS-PAGE to verify protein loading (lower) for control cells and three LCIP63-overexpressing clones. b Rate of net photosynthesis versus concentration of DIC. EV corresponds to the clone transformed with an empty vector; C1, C2, C3, to clones 1, 2, and 3 overexpressing LCIP63. Theoretical curves showing a close-up of fits obtained with experimental data (3 replicates). Fits were performed using Michaelis–Menten equation; dashed vertical lines represent the mean of K0.5 [DIC] that are indicated in parentheses. All parameters and statistical analysis are given in Table 1
