Fig. 4
From: A new widespread subclass of carbonic anhydrase in marine phytoplankton
Characterization of two oligomeric forms of LCIP63. a Elution profile of LCIP63 using size-exclusion chromatography. Arrows indicate elution volumes of proteins used for calibration: 0, Void volume/Blue dextran (2000 kDa); 1, ferritin (440 kDa); 2, catalase (232 kDa); 3, glyceraldehyde-3-phosphate dehydrogenase (150 kDa); 4, bovine serum albumin (68 kDa); 5 ovalbumin (45 kDa). b CA activity of the high molecular mass form (HMM, eluted at 56 ml) and the low molecular mass form (LMM, eluted at 64 ml). c SDS-PAGE of LCIP63 (2 µg): (1) ladder, (2) HMM fraction, (3) LMM fraction. d Schematic representation of the primary structure of LCIP63 from six diatom species. Yellow and green boxes represent ER transit and chloroplast targeting peptides, respectively, light blue boxes represent CaMKII-AD and dark blue boxes the ‘(H)HHSS’ motif. e, f CA activity of the high molecular mass (HMM) and low molecular mass (LMM) forms of the LCIP63 variant containing three (e) and two (f) domain repeats. **p ≤ 0.01; ***p ≤ 0.001. Error bars show mean ± SD, n = 3–6
