Fig. 5

Anaerobic hydrocarbon degradation inferred from JS1 MAGs associated with hydrocarbon-enriched environments. Pathways with genes detected were depicted in solid black lines, pathway with genes not found in all MAGs were depicted in grey lines. The ATP production/consumption in putative reaction catalyzed by assK and pccA were predicted resembling well-defined reaction of acetyl-CoA ligation and methylmalonyl-CoA decarboxylation. ΔG° standard Gibbs free energy was calculated based on reactants and products at 1 M concentration and gases at a partial pressure of 1atm. Gibbs free energy of formation for fumarate in the liquid state was taken from NIST Chemistry WebBook (https://webbook.nist.gov/chemistry/), all other compounds are in the aqueous phase