Fig. 4: NanoSIP single cell and moss tissue enrichment for three stable isotopes over time.

NanoSIMS-derived 15N APE (a, b), 13C APE (c, d), and 33S APE (e, f) values for single Nostoc M2 cells (left) and moss tissue (right), at three successive time points following colonization. “Control” indicates treatment wherein both moss and Nostoc M2 were chemically killed before incubation. Brackets and star indicates significant (p < 0.05) difference from control. (e) Ratios have been background corrected for contrition of the highly labeled moss tissue. Different color hues in each column represent different moss phyllids (b, d, f) or single cells (a, c, e) originating from different phyllids. Each point represents a single cell or tissue measurement, and error bars represent one standard error of the mean (n values on graph).